FIGURE 6.

Effects of PDI overexpression on HIF-1α-mediated gene expression. GH3 cells were cultured under normoxia (Nor) or hypoxia (Hyp) (1% O₂, 5% CO₂) for 6 h. Total RNA was isolated in three culture plates, and mRNA levels of VEGF were determined by qRT-PCR. VEGF mRNA levels of mock-transfected cells without T₃ were set at 1.0. Values are expressed as the mean ± S.D. (error bars) for three replicates. **, p < 0.01, significantly different from control.