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. 2012 Nov 12;288(3):1706–1716. doi: 10.1074/jbc.M112.365239

TABLE 1.

Primers for cloning of Ref-1 and TRs

Restriction sites are underlined, and start/stop codons are double underlined.

Factor Primer no. Sequence
TRα 1 AAGAATTCGTGAATGGAACAGAAG
2 TTCTCGAGTTAGACTTCCTGATC
TRβ1 3 AAGAATTCAACCTATGACTCCTAA
TRβ2 4 AAGAATTCTGTTCATGTGTATGGAT
TRβ1/2 5 TTCTCGAGTCAGTCCTCAAAGACTT
Ref-1 6 AAGGATCCTTCGTTACAGCGATGCCG
7 ATCCACATTCCAGGAGGCTATCTTGAGTGT
8 TCCTGGAATGTGGATGGGCTTCGA
9 TTCTCGAGTGAGGGGAGTGTCACAGT
10 TTGTCGACGCTGAGGGGAGTGTCACAGT
TRX1 11 AAGGATCCATGGTGAAGCTGATCGAGAG
12 TTCTCGAGGCATGATTAGGCAAACTCCGTAATAGTG