FIGURE 6.

Release of the wild type yeast cells from α-factor block stimulates Kcs1 activity. Cells were labeled, synchronized in G₁ with α-factor, released into fresh medium, and harvested at the indicated times. Inositol phosphates were analyzed by HPLC, whereas Kcs1 activity was assayed using InsP₆ as the substrate as described under “Experimental Procedures.” Shown are the time course of changes in the level of InsP₆ (A), InsP₇ (B), and InsP₈ (C) and Kcs1 activity (D) after the release of the cells from α-factor block. The results are means ± S.E. (error bars) for three different experiments. *, p < 0.05 (Student's t test) with respect to time 0.