FIGURE 8.

Changes in inositol phosphate levels in kcs1Δ cells following the release from α-factor block. Cells were labeled, synchronized in G₁ with α-factor, released into fresh medium, and harvested at the indicated times, and inositol phosphates were analyzed by HPLC as described under “Experimental Procedures.” HPLC elution profile of inositol polyphosphates at time 0 (A) or 30 min after the release of the cells from α-factor block (B). Time course of changes in the level of InsP₃ (C) and InsP₆ (D) after the release of the cells from α-factor block. The results are means ± S.E. for three different experiments. *, p < 0.05 (Student's t test) with respect to time 0.