FIGURE 10.

Effects of D11Q mutation on the conformation of the DNase loop in monomeric actin. A, time course of the subtilisin (sub) cleavage of monomeric WT and D11Q actins in G-buffer, as assayed by SDS-PAGE and densitometry of the stained gel. Inset, SDS-PAGE of WT and D11Q actins at 0 (control) and 2.5 min (+sub) of incubation with 1 μg/ml subtilisin. B, inhibitory effect of WT and D11Q actins on the activity of DNase I. Student's t test on three independent sets of data indicated that the difference is significant with p = 0.016.