FIGURE 3.

Electron micrographs of negatively stained actin polymers. WT (A and B), D11Q (C and D), and D11N (E and F) actins were polymerized in F-buffer in the absence (A, C, E, and F) or presence (B and D) of 20 μm phalloidin (Ph) for 2 h, diluted, and stained with uranyl acetate. Arrowheads indicate oligomeric structures in D11Q polymers that appear associated along the length of filaments. F is a gallery of D11N rings. Bars, 50 nm, except for F (25 nm).