FIGURE 8.

Cofilin-induced depolymerization. A, 5 μm WT actin filaments and a 1:1 mixture of WT and D11Q actin polymers were treated with 10 μm cofilin at pH 8.3, and after incubation for 15 min, the mixtures were subjected to ultracentrifugation followed by SDS-PAGE of the supernatant (sup) and pellet fractions. 2.5 μm WT actin was labeled with Alexa-Fluor 488 (Fluor). Fluorogram visualized WT subunits only and Coomassie Brilliant Blue (CBB) stained both WT and mutant actins. B, fluorescence microscopic observation of cofilin-induced depolymerization of Alexa-Fluor 488-labeled WT filaments and 1:1 copolymer of labeled WT and unlabeled D11Q actin. Bar, 20 μm.