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. 2012 Dec 7;288(3):1750–1761. doi: 10.1074/jbc.M112.397604

FIGURE 1.

FIGURE 1.

Developmental expression profiles of B. malayi C-P4H genes assessed by RT-PCR. For each gene, simultaneous amplifications were performed using gene-specific primers and primers to B. malayi tubulin (Bma-tub-1) (accession no. AJ551180) as an internal control. A, expression of Bma-phy-2, Bma-pdi-2, and Bma-phy-1 in microfilaria (first stage larvae). B, expression of Bma-phy-2 and Bma-pdi-2 in post-infective stages. Parasite material was taken from the time points indicated post-infection (days pi) from infected jirds. L3 and L4 denote larval stages 3 and 4, and Adult indicates juvenile adults. Sizes of cDNA amplicons were as follows: Bma-phy-2, 808 bp; Bma-pdi-2, 597 bp; Bma-phy-1, 655 bp; Bma-tub-1, 308 bp. Sizes of genomic amplicons, Bma-phy-2, 3529 bp; Bma-pdi-2, 1301 bp; Bma-phy-1, 1909 bp; Bma-tub-1, 390 bp.