FIGURE 6.

Western blots (reducing SDS-PAGE) of insect cell extracts compared with B. malayi adult worms. Lanes 1, 3, and 5 are extracts from insect cells co-expressing Bma-PHY-1, Bma-PHY-2, and Bma-PDI-2 (3XBma). Lanes 2, 4, and 6 are B. malayi extracts (Adult Bma). The antibodies used are indicated at the side of each blot. All three antibodies detect a band of the same size, running at approximately the size expected of a dimer (migrating at approximately 100 kDa), in the B. malayi worm extracts only. The sizes of the mature (minus signal peptide) proteins are as follows: Bma-PHY-1, 60 kDa; Bma-PHY-2, 62 kDa; Bma-PDI-2, 54 kDa.