FIGURE 4.

IQD1 and IQD20 interact with Arabidopsis CaM/CMLs in the yeast two-hybrid system. Yeast cells containing the indicated combinations of IQD1, IQD20, or CNGC2 cDNAs in the bait Gal4 DNA binding domain vector (panels BD-IQD1, BD-IQD20, BD-CNGC2, and BD-empty for control) and different CaM or CML cDNAs as well as IQD1 or IQD20 cDNAs in the prey Gal4 activation domain vector (listed in center AD column) were grown in serial 10-fold dilutions on (−L−W) SD medium to select for the presence of both vectors and on growth restrictive (−L−W−H) SD medium to test for interaction of the encoded fusion proteins (note, the upper panels are composites from different but representative agar plates). The left and right columns list the corresponding values (Miller units) of quantitative yeast two-hybrid interaction assays (β-galactosidase reporter activity). Qualitative and quantitative interaction assays were repeated 3–5 times giving similar results.