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. 2012 Nov 30;288(3):1871–1882. doi: 10.1074/jbc.M112.396200

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FIGURE 5. — IQD1 interacts with KLCR1 and GSTU26 in the yeast two-hybrid system. Confirmation of yeast two-hybrid interactions of IQD1 with KLCR1 (upper panels) and of IQD1 with GSTU26 (lower panels) is shown. Interaction assays between different test and control constructs were performed by co-transformation into yeast cells. Colony growth and lacZ expression (+X-gal) was compared on (−L−W) SD media to select for bait Gal4-BD and prey Gal4-AD vectors (left panels) and on growth restrictive (−L−W−H−Ade) SD media to test for protein interaction (right panels). Numbers (1–8, referring to all plates) indicate the following vector combinations: 1, BD-KLCR1/AD-IQD1 or BD-GSTU26/AD-IQD1; 2, BD-KLCR1/AD-empty or BD-GSTU26/AD-empty; 3, BD-IQD1/AD-KLCR1 or BD-IQD1/AD-GSTU26; 4, BD-empty/AD-KLCR1 or BD-empty/AD-GSTU26; 5, BD-CNGC2/AD-CaM1; 6, BD-empty/AD-KLCR1 or BD-empty/AD-GSTU26; 7, BD-IQD1/AD-CaM1; 8, BD-CaM1/AD-IQD1.