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. 2012 Dec 3;288(3):1967–1978. doi: 10.1074/jbc.M112.407205

FIGURE 2.

FIGURE 2.

Most genes in the innate immunity protein interaction network affect LPS-induced IL-6 production in the J774A.1 mouse macrophage cell line. A, pools of four siRNA duplexes per gene were transfected into the mouse macrophage cell line J774A.1; cells were stimulated with 20 ng/ml LPS for 6 h, and IL-6 production was monitored by ELISA on cell supernatants. IL-6 production was normalized relative to a control pool of siRNA duplexes (CT1, Dharmacon nontargeting siRNA pool). CT2 is a second negative control (Dharmacon nontargeting siRNA 1). TLR4, the LPS receptor, is presented as a positive control. Two genes in this network (Macf1 and Siah1a) were inhibited previously; the data for these two genes from this prior publication (12) is presented at the end of the panel. B depicts the effects on viability of the indicated siRNA treatments normalized so that viability of control siRNA was equal to 1. C, depicts the results of qPCR, which was used to monitor RNA knockdown of the indicated genes. Asterisks indicate siRNA treatments that induced IL-6 levels (A) or gene knockdown (C) that were significantly different from the controls (p < 0.05). No viability measurements (B) were statistically significantly different from control.