TABLE 1.
B. subtilis strains used in this study
| Strain | Genotype | Source /Ref.a |
|---|---|---|
| 168 | trpC2 | Laboratory collection |
| GP193 | trpC2 Ωrny::pGP774 (pxylA-rny cat) | 50 |
| GP983 | trpC2 ΔcdaS::ermC | LFH-PCR → 168 |
| GP985 | trpC2 ΔcdaAR::cat | LFH-PCR → 168 |
| GP987 | trpC2 ΔdisA::tet | YA5 → 168 |
| GP989 | trpC2 ΔcdaS::ermC ΔcdaAR::cat | GP983 → GP985 |
| GP991 | trpC2 ΔcdaS::ermC ΔdisA::tet | GP983 → GP987 |
| GP997 | trpC2 ΔcdaA::cat | LFH-PCR → 168 |
| GP998 | trpC2 ΔgdpP::spc | YA188 → 168 |
| GP999 | trpC2 ΔcdaR::cat | LFH-PCR → 168 |
| GP1026 | trpC2 cshA-Strep aphA3 | 53 |
| GP1173 | trpC2 lacA:: (pxylA yfpC aphA3) | 23 |
| GP1319 | trpC2 amyE::lacZ cat | pAC6 → 168 |
| GP1323 | trpC2 lacA:: (pxylAcdaS aphA3) ΔdisA::tet ΔcdaS::ermC | pGP1959 → GP991 |
| GP1327 | trpC2 lacA:: (pxylAcdaS aphA3) ΔdisA::tet ΔcdaS::ermC ΔcdaA::cat | GP997 → GP1323 |
| GP1328 | trpC2 lacA:: (pxylAcdaS aphA3) ΔdisA::tet ΔcdaS::ermC ΔcdaR::cat | GP999 → GP1323 |
| GP1329 | trpC2 lacA:: (pxylAcdaS aphA3) ΔdisA::tet ΔcdaS::ermC ΔcdaAR::cat | GP985 → GP1323 |
| GP1330 | trpC2 lacA:: (cdaR-Strep aphA3) | pGP1969 → 168 |
| GP1331 | trpC2 lacA:: (cdaR-Strep aphA3) cdaA-3xFLAG ermC | pGP1966 → GP1330 |
| GP1332 | trpC2 lacA:: (cdaR-Strep aphA3) cshA-3xFLAG spc | pGP1333 → GP1330 |
| GP1333 | trpC2 cshA-Strep aphA3 cdaA-3xFLAG ermC | pGP1966 → GP1026 |
| GP1334 | trpC2 lacA:: (pxylAcdaSL44F aphA3) ΔdisA::tet ΔcdaS::ermC ΔcdaA::cat | Suppressor mutant picked from GP1327 on a plate without xylose |
| GP1339 | trpC2 amyE:: (cdaA-lacZ cat) | pGP1980 → 168 |
| GP1340 | trpC2 amyE:: (glmM-lacZ cat) | pGP1981 → 168 |
| GP1341 | trpC2 lacA:: (pxylAcdaS aphA3) | pGP1959 → 168 |
| GP1342 | trpC2 lacA:: (pxylAcdaSL44F aphA3) | GP1334 → 168 |
| GP1343 | trpC2 lacA:: (pxylAcdaS aphA3) ΔgdpP::spc | pGP1959 → GP998 |
| GP1344 | trpC2 lacA:: (pxylAcdaSL44F aphA3) ΔgdpP::spc | GP1334 → GP998 |
| GP1346 | trpC2 ΔcdaS::ermC ΔdisA::tet ΔcdaR::cat | GP999 → GP991 |
| GP1348 | trpC2 lacA:: (pxylA-m cdaS aphA3) ΔdisA::tet ΔcdaS::ermC ΔcdaA::cat | Suppressor mutant picked from GP1327 on a plate without xylose |
| YA5 | ΔdisA::tet | 38 |
| YA188 | ΔgdpP::spc | 8 |
a Arrows indicate construction by transformation.