TABLE 3.

Control of diadenylate cyclase activities by the regulatory protein CdaR

Diadenylate cyclase	Plasmid	ng c-di-AMP/mg proteina,b
		−CdaR	+CdaR (pGP1984)
None	Empty vector	0 (0)	0 (0)
CdaA	pGP1970	237 (72)	5,256 (1,038)
CdaS	pGP1972	562 (140)	342 (22)
DisA	pGP2563	79,187 (25,875)	70,511 (2,313)

^a E. coli cultures harboring the relevant plasmids were grown in LB medium, and cell extracts were prepared as described under “Experimental Procedures.” c-di-AMP levels were determined by HPLC-MS/MS analysis.

^b Mean values from three individual experiments were calculated. Values in parentheses indicate the S.D.