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. 2012 Nov 27;288(3):2018–2028. doi: 10.1074/jbc.M112.428847

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — TraM localization and characterization. A, TraM localizes to the cell envelope of pIP501 harboring E. faecalis JH2-2 cells. The localization of TraM in the cell fractions was detected by Western blot with anti-TraMΔ antibodies. CW, cell wall; M, membrane; CP, cytoplasm. B, cross-linking assay of TraMΔ. Lane M, molecular mass standard; lane 1, control (no glutardialdehyde, no treatment); lanes 2–6, TraMΔ with 0/0.001/0.01/0.05/0.1% glutardialdehyde. C, opsonophagocytic killing and inhibition of killing assays using anti-TraMΔ antisera.