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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: ACS Chem Biol. 2012 Apr 13;7(6):961–966. doi: 10.1021/cb300056q

Figure 2.

Figure 2

Preparation of crude IJ pheromone and activity-guided fractionation using the IJ recovery assay. (A) IJs were placed on a P. luminescens lawn on the lipid-agar side of a split petri plate. The IJs developed into adults, which laid eggs for several days but then underwent endotokia matricida, generating IJs that dispersed and got trapped in the saline solution. The IJs were collected on day 7–8 for use in the IJ recovery assay, and lipid-agar was harvested on day 14 for extraction to generate crude IJ pheromone. (B) The IJ recovery assay was used in activity-guided fractionation of the crude IJ pheromone. IJs were placed on a thin lawn of P. luminescens on small lipid-agar petri plates, and recovery was observed in the presence and absence of pheromone.