Figure 1.
φX216 one-step growth curve. φX216 was adsorbed to B. mallei ATCC23344 cells for 15 min, inoculated into LB + 2% glycerol, and cultures were incubated at 37°C with shaking. Triplicate aliquots were removed at the indicated time intervals and used to inoculate plaque plates to determine pfu/mL. The pfu/mL values were divided by the means of the T0 and T1 (1 h) phage concentrations to adjust to pfu/input pfu.