Table 1.
B. pseudomalleiP2-like prophage distribution screen
| |
|
P2-like prophage PCR probe results |
|||||
|---|---|---|---|---|---|---|---|
| |
|
Multiplex |
|
|
|
||
| B. pseudomallei | Candidate P2-like prophage group a | P2-like 1 | P2-like 2 | P2-like group A | φX216 scrnA | φX216 scrnB | GI2 |
|
Strains with high φX216 plaquing efficiency b 66.7% (22/33) P2-like prophage candidate positive strains |
|
||||||
| 2668a |
φ52237-like |
+ |
+ |
+ |
+ |
+ |
+ |
| E0237 c |
φ52237-like |
+ |
+ |
+ |
+ |
+ |
+ |
| E0394 |
φ52237-like |
+ |
+ |
+ |
+ |
+ |
+ |
| 1026b d |
φ52237-like |
+ |
+ |
+ |
+ |
+ |
+ |
| 708a |
φ52237-like e |
+ |
+ |
+ |
+ |
- |
+ |
| 2618a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2661a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2692a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2717a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0021 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0235 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0279 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0345 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0384 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0386 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| K96243 f |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| S13 g |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2698a |
P2L |
+ |
+ |
- |
- |
- |
- |
| 2704a |
P2L h |
+ |
+ |
- |
- |
+ |
- |
| E0342 |
P2L |
+ |
+ |
- |
- |
- |
- |
| E0366 |
P2L |
+ |
+ |
- |
- |
- |
- |
| E0377 |
P2L |
+ |
+ |
- |
- |
- |
- |
| 2613a |
|
- |
- |
- |
- |
- |
ND i |
| 2667a |
|
- |
- |
- |
- |
- |
ND |
| 2673a |
|
- |
- |
- |
- |
- |
ND |
| 2682a |
|
- |
- |
- |
- |
- |
ND |
| 2769a |
|
- |
- |
- |
- |
- |
ND |
| E0016 |
|
- |
- |
- |
- |
- |
ND |
| E0034 |
|
- |
- |
- |
- |
- |
ND |
| E0241 |
|
- |
- |
- |
- |
- |
ND |
| E0356 |
|
- |
- |
- |
- |
- |
ND |
| E0411 |
|
- |
- |
- |
- |
- |
ND |
| MSHR305 |
|
- |
- |
- |
- |
- |
ND |
|
Strains with low φX216 plaquing efficiency j 17.4% (4/23), P2-like prophage candidate positive strains |
|
||||||
| 2625a |
φ52237-like |
+ |
+ |
+ |
+ |
+ |
+ |
| 2670a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0037 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| E0380 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2637a |
|
- |
- |
- |
- |
- |
ND |
| 2650a |
|
- |
- |
- |
- |
- |
ND |
| 2660a |
|
- |
- |
- |
- |
- |
ND |
| 2685a |
|
- |
- |
- |
- |
- |
ND |
| 2708a |
|
- |
- |
- |
- |
- |
ND |
| 2719a |
|
- |
- |
- |
- |
- |
ND |
| 2764b |
|
- |
- |
- |
- |
- |
ND |
| E0024 |
|
- |
- |
- |
- |
- |
ND |
| E0031 |
|
- |
- |
- |
- |
- |
ND |
| E0181 |
|
- |
- |
- |
- |
- |
ND |
| E0371 |
|
- |
- |
- |
- |
- |
ND |
| E0372 |
|
- |
- |
- |
- |
- |
ND |
| E0378 |
|
- |
- |
- |
- |
- |
ND |
| E0383 |
|
- |
- |
- |
- |
- |
ND |
| E0393 |
|
- |
- |
- |
- |
- |
ND |
| 1710a |
|
- |
- |
- |
- |
- |
ND |
| 1710b k |
|
- |
- |
- |
- |
- |
- |
| 1106b |
|
- |
- |
- |
- |
- |
ND |
| 406e |
|
- |
- |
- |
- |
- |
ND |
|
Non-φX216 plaquing strains 25.0% (4/16), P2-like prophage candidate positive strains |
|
||||||
| 2671a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2674a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2677a |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| Pasteur 6068 |
P2L-A |
+ |
+ |
+ |
- |
- |
+ |
| 2614a |
|
- |
- |
- |
- |
- |
ND |
| 2617a |
|
- |
- |
- |
- |
- |
ND |
| 2640a |
|
- |
- |
- |
- |
- |
ND |
| 2665a |
|
- |
- |
- |
- |
- |
ND |
| 2689b |
|
- |
- |
- |
- |
- |
ND |
| 2694a |
|
- |
- |
- |
- |
- |
ND |
| E0008 |
|
- |
- |
- |
- |
- |
ND |
| E0183 |
|
- |
- |
- |
- |
- |
ND |
| E0350 |
|
- |
- |
- |
- |
- |
ND |
| E0396 |
|
- |
- |
- |
- |
- |
ND |
| 1106a |
|
- |
- |
- |
- |
- |
ND |
| MSHR668 | - | - | - | - | - | ND | |
a φ52237-like assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A, and individual PCR probes φX216 scrnA, φX216 scrnB and GI2. P2L-A assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A and individual PCR probe GI2. P2L assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2.
bConfluent lysis when spot tested with ~106 pfu φX216.
cφX216 source strain.
d1026b φ52237-like prophage is split into two segments and likely non-functional [15].
eP2-like prophage group cannot be determined based on PCR results. May be P2L-A or φ52237-like.
fφK96243 prophage (group P2-A) located at GI2 [9].
gEncodes the predicted prophage PI-S13-1 (group P2-A) [88].
hP2-like prophage group cannot be assigned based on PCR results. May be P2L or φ52237-like.
IND, GI2 probe results not determined.
jNon-confluent lysis / individual plaques when spot tested with ~ 106 pfu φX216.
kThe strain 1710b genome does not contain a P2-like prophage or prophage insertion at GI2.