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. 2012 Oct 11;31(1):85. doi: 10.1186/1756-9966-31-85

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Copyright ©2012 Matsutani et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Serum antibody levels of glioma SEREX antigens. cDNA inserts of identified clones were recombined in-frame into pGEX vectors that express recombinant GST fusion proteins. Using the fusion proteins as antigens, the levels of antibodies were examined by the ELISA and shown by the ordinate, as (A) CENPF, (B) MIF, (C) M-RIP, (D) RBBP8, (E) RPLP0, (F) TAF7, (G) TLN1, (H) TGFBI, (I) UNC45A. The significance of differences among healthy donors, patients with low-grade glioma and with high-grade glioma was calculated using Kruskal Wallis H-test and Mann–Whitney U-test with Bonferroni correction. The box-and-whisker plots display the 10th, 25th, 50th, 75th and 90th percentiles.