Figure 5.

The detection of epitope site by overlapped peptide array. Series of peptides of 14 amino acid residues, composed of SH3GL1, were synthesized with overlapping by 12 amino acids, and were blotted in nitrocellulose membranes using F-moc amino acids (A). Three sera of the patients with low-grade glioma indicated the fine reaction in spot 177 and 178 (C), compared to two normal volunteers (D) and no serum control (B). The calculated fluorescence intensity, normalized by background control, revealed that these spots were suggested as a minimum epitope site (E).