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. 2013 Jan 6;14:1. doi: 10.1186/1471-2091-14-1

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Copyright ©2013 Kobayashi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIP200 interacts with COP1. (A) Y190 yeast cells were transfected with pAS2 vectors containing full-length and deletion mutants (ΔWD40 and ΔRING) of COP1 together with pACT vectors containing FIP200 and JunD. Transfectants were grown on a Trp^- Leu^- His^- selection medium and tested for β-galactosidase activity (top). Schematic structure of COP1 mutants (bottom). (B) GST and GST-FIP200 recombinant proteins were immobilized on glutathione beads (bottom panel, separated by SDS-PAGE and stained with CBB) and mixed with cell lysate containing ectopically expressed HA-tagged wild-type and ΔWD40-mutant form of COP1. Bound proteins were analyzed by Western blotting using antibody against COP1 (upper panel).