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. 2013 Jan 18;8(1):e54382. doi: 10.1371/journal.pone.0054382

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© 2013 Koreishi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) The predicted domain structure of human Sec31 by SMART (http://smart.embl-heidelberg.de). The domain structure of Sec31 is: N-terminal WD repeats for Sec13 binding, a linker region, and a C-terminal extensin-like proline-rich domain for Sec23 binding. The phosphorylation sites identified by mass-spectrometry were indicated by arrows. (B) HEK293 cells were transfected with FLAG-tagged wild type (WT) Sec31 or its S527A/S799A/S1163A/T1165A mutant (4SA). Tagged proteins were immunoprecipitated with antibodies to the FLAG tag and subjected to western blotting with anti-FLAG and anti-phospho-serine/threonine antibodies. The immunoprecipitated (IP’ed) phospho-Sec31 levels were normalized with IP’ed Sec31 levels and expressed as the normalized ratio.