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. 2013 Jan 18;8(1):e54467. doi: 10.1371/journal.pone.0054467

Figure 1. The process of screening and enriching the binding peptide library for cancer cells with bacteria surface display methods.

Figure 1

(A) Schematic representation of peptides screening and selection with bacterial display library. 1. Constructed the library by transforming plasmids into E.coli MC1061; 2. Discarded the bacteria binding with normal cells after pre-incubation; 3. Incubated the mixture of cancer cells and residual bacteria; 4. Analyzed the binding effect of cancer cells with bacteria using FACS; 5. Sorted the binding bacteria to cancer cells by flow cytometry and cultured the binding bacteria in medium; 6. Performed the next round binding bacteria screening; 7. Isolated the binding bacteria to cancer cells and sequenced the peptides displayed on the surface of the bacteria. (B) The progress of enrichment of bacteria binding with cancer cells by FACS in 6 screening rounds.