Figure 6. Drug and nanoparticles uptake by spheroids.

A) Representative images of Doxorubicin uptake in H1650 parental cell spheroids grown in 3D alginate scaffold system Uptake Images were taken at 0.5, 1 and 2 h time points, B) Representative Images of the nanoparticle uptake by H1650 parental cell spheroids. DIO oil was encapsulated in NLC and incubated with the spheroids for 2 hours, a) Fluorescent image, b) DIC image and c) merged image. The fluorescent images clearly indicates the nanoparticles uptake into spheroids, C) Relative fluorescence intensities of free Doxorubicin uptake and DIO oil loaded NLC nanoparticles into 3D spheroids. Each data point is represented as mean ± sem (n = 3). **P<0.01 Vs Doxorubicin group.