Skip to main content
. 2013 Jan 18;8(1):e54303. doi: 10.1371/journal.pone.0054303

Table 1. Primers for RT-PCR.

Molecules MW Locus Primer sequence Tm °C
VEGFR2 123 NM_013062 F 5′-ATCGGTGAGAAAGCCTTGATCTC-3′ 53
R 5′-TTCTAGCTGCCAGTACCATTGGA-3′
CD133 97 NM_001110137 F 5′-CTGCAAACCCATGATTACAGCAA-3′ 57
R 5′-CCCTATGCCGAACCAGAACAG-3′
SDF-1α 240 NM_022177 F 5′-TCTTTGGCCTCCTGTAGAATGG-3′ 56
R 5′-TCACGGCAAGATTCTGGCTTA-3′
CXCR4 177 NM_001033882 F 5′-CGTGAATGAGTGTCTAGGCAGG-3′ 55
R 5′-GGCTTTGGTTTTAAGTGCCATC-3′
ACTIN 94 NM_0010822 F 5′-AGACCTTCAACACCCCAG-3′ 55
R 5′-CACGATTTCCCTCTCAGC-3′

VEGFR2, vascular endothelial growth factor receptor 2; SDF-1α, stromal cell–derived factor-1α; CXCR4, chemokine (C-X-C motif) receptor 4; MW, molecular weight; Tm, melting temperature.