Table 1. Primers for RT-PCR.
Molecules | MW | Locus | Primer sequence | Tm °C |
VEGFR2 | 123 | NM_013062 | F 5′-ATCGGTGAGAAAGCCTTGATCTC-3′ | 53 |
R 5′-TTCTAGCTGCCAGTACCATTGGA-3′ | ||||
CD133 | 97 | NM_001110137 | F 5′-CTGCAAACCCATGATTACAGCAA-3′ | 57 |
R 5′-CCCTATGCCGAACCAGAACAG-3′ | ||||
SDF-1α | 240 | NM_022177 | F 5′-TCTTTGGCCTCCTGTAGAATGG-3′ | 56 |
R 5′-TCACGGCAAGATTCTGGCTTA-3′ | ||||
CXCR4 | 177 | NM_001033882 | F 5′-CGTGAATGAGTGTCTAGGCAGG-3′ | 55 |
R 5′-GGCTTTGGTTTTAAGTGCCATC-3′ | ||||
ACTIN | 94 | NM_0010822 | F 5′-AGACCTTCAACACCCCAG-3′ | 55 |
R 5′-CACGATTTCCCTCTCAGC-3′ |
VEGFR2, vascular endothelial growth factor receptor 2; SDF-1α, stromal cell–derived factor-1α; CXCR4, chemokine (C-X-C motif) receptor 4; MW, molecular weight; Tm, melting temperature.