Figure 1. AHK5 interacts with a set of AHP proteins in yeast-two-hybrid assays. (A) Yeast-two-hybrid assay with yeast cells co-expressing BD::AHK5 (AHK5 protein fused to the BD domain of the Gal4 DNA) and the indicated AD::AHP (AHP1 to 5 proteins fused to the AD domain of the Gal4 DNA) fusion proteins. Yeast cells were incubated for 4 d at 28°C on either vector selective (L-, W-) or interaction selective media (L-, W-, Ade-) (B) Western-blot analysis using crude extracts from transformed yeast cells co-expressing the indicated AD::AHP (AHP1 to 5) and BD::AHK5 fusion proteins. The AD::AHP (upper panel) and the BD::AHK5 (lower panel) fusions were detected with a HA- and c-myc-specific antibody respectively.