Figure 2. AHK5 interacts with a set of AHP proteins in a Bimolecular Fluorescence Complementation Assay (BiFC). (A) Confocal images of epidermal tobacco leaf cells (Nicotiana benthamiana) co-expressing the indicated YFP-N and YFP-C fusion proteins. The left panels show the fluorescence signal, the middle panels the bright field images of identical cells and the right panels the overlay of both. YFP-N, N-terminal YFP fragment fused to the different AHP proteins; YFP-C, C-terminal YFP fragment fused to the AHK5 protein. The bars represent 10 μm. (B) Western-blot analysis using crude protein extracts from transiently transformed tobacco leaves analyzed for BiFC before extraction. The AHP::YFP-N (upper panel) and the AHK5::YFP-C (lower panel) fusions were detected with a c-myc and HA-specific antibody respectively. M, protein marker.