Table 2.

Steady-State Kinetic Parameters for Wild-type Fmsl and the N195A and D94N Enzymes^a

substrate	kinetic parameter	Fms1d	N195A	D94N
N1-acetylspermineb	kcat (s-1)c	15.1 ± 0.4	8.1 ± 0.2	9.7 ± 0.2
	kcat/Kamine (mM-1 s-1)c	1400 ± 200	78 ± 13	320 ± 50
	Kamine (μM)c	10.9 ± 1.8	104 ± 14	30 ± 5
	kcat/KO2 (mM-1 s-1)e	358 ± 20	405 ± 72	100 ± 14
	KO2 (μM)e	43.6 ± 2.3	20 ± 3	97 ± 14
sperminef	kcat (s-1)c	39.0 ± 1.5	4.9 ± 0.2	2.0 ± 0.3
	kcat/Kamine (mM-1 s-1)c	330 ± 60	38.5 ± 8.3	4.1 ± 1.0
	Kamine (μM)c	118 ± 25	127 ± 27	320 ± 88
	kcat/KO2 (mM-1 s-1)g	428 ± 77	327 ± 67	104 ± 36
	KO2 (μM)g	91 ± 16	15 ± 3	192 ± 60

^aConditions: 25 °C.

^bDetermined at pH 9.0.

^cDetermined by varying the concentration of the amine at 1.2 mM oxygen.

^dFrom ref. (13).

^eDetermined by varying the concentration of oxygen at 20 mM N¹-acetylspermine.

^fDetermined at pH 9.35.

^gDetermined by varying the concentration of oxygen at 20 mM spermine.