Table 4.
substrate | kinetic parameter | Fms1b | N195A | D94N | H67Qb |
---|---|---|---|---|---|
N1-acetylspermine | Kd (μM) | 484 ± 83 | 60 ± 10 | 73 ± 17 | 23 ± 1 |
k2 (s-1) | 5490 ± 90 | 9.0 ± 0.2 | 15.6 ± 0.7 | 58 ± 3 | |
spermine | Kd (μM) | 23 ± 8 | 84 ± 20 | 282 ± 38 | 3300 ± 500 |
k2 (s-1) | 126 ± 3 | 16.4 ± 0.6 | 6.10 ± 0.25 | 2.1 ± 0.5 |
Determined at pH 9.0, 25 °C.
From ref. (11).