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. Author manuscript; available in PMC: 2013 Oct 30.
Published in final edited form as: Biochemistry. 2012 Oct 15;51(43):8690–8697. doi: 10.1021/bi3011434

Table 4.

Rapid Reaction Kinetic Parameters for Fmsl Wild-Type and Mutant Enzymesa

substrate kinetic parameter Fms1b N195A D94N H67Qb
N1-acetylspermine Kd (μM) 484 ± 83 60 ± 10 73 ± 17 23 ± 1
k2 (s-1) 5490 ± 90 9.0 ± 0.2 15.6 ± 0.7 58 ± 3
spermine Kd (μM) 23 ± 8 84 ± 20 282 ± 38 3300 ± 500
k2 (s-1) 126 ± 3 16.4 ± 0.6 6.10 ± 0.25 2.1 ± 0.5
a

Determined at pH 9.0, 25 °C.

b

From ref. (11).