qRT-PCR results for A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5), matrix metalloproteinase 3 (MMP3), matrix metalloproteinase 9 (MMP9), and Interleukin 1 Beta (IL1β). Student's t-test indicated significant differences from day 0 represented by a *. A two way ANOVA was performed with degeneration and media condition as factors, with the results shown in the upper right corner for each gene. Treatment groups with different letters are significantly different (Fisher's post hoc test α=0.05 was run for each media condition with pooled degenerative grades). ∼ indicates the gene was not expressed by that group. Significance was always defined as p<0.05, error bars represent SEM.