Table 1.

Chk1 and Wee1 inhibitors combine synergistically with chemotherapy, and each other, in neuroblastoma cells. Neuroblastoma cell lines treated with a combination of inhibitor and chemotherapy, or a combination of Chk1 and Wee1 inhibitor together demonstrated cytotoxic synergy.

Cell Line	MK-8776 + SN38	MK-1775 + SN38	MK-8776 + MK1775	MK-8776 + Gemcitabine	MK-1775 + Gemcitabine
IMR-5	±	+	+++++	+++	+++++
BE2C	±	+++	++	++++	+++
NB1	++	−−	−−	+++	−−−
Ebc1	+++++	±	+++++	+++++	++++
NBSD	+++	++++	+++	+++++	±
Kelly	+++++	+++++	++	++++	+++++
NGP	++++	+++	++++	+++++	+++++
SKNAS	++++	+++++	+++	+++++	+++++
NLF	++++	+++	++++	+++++	−
NB-1643	+++	+++	+++++	+++++	+++++

CI Value	Symbol	Observed Synergy
<0.1	+++++	very strong
0.1–0.3	++++	strong
0.3–0.7	+++	synergy
0.7–0.85	++	moderate synergism
0.85–0.9	+	slight synergism
0.9–1.1	±	additive
>1.1	−	antagonistic