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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Dev Biol. 2012 Nov 30;374(1):223–233. doi: 10.1016/j.ydbio.2012.11.021

Fig. 1. Expression of plet-7::GFP reporters.

Fig. 1

(A) Diagram of the endogenous let-7 gene and reporter constructs. The plet-7A::GFP and plet-7B::GFP constructs contain 752 and 1568 nt of endogenous let-7 promoter, respectively, fused to GFP with a nuclear localization signal (NLS) and the let-858 3’UTR. (B) The spatio-temporal expression pattern of plet-7::GFP in transgenic animals was analyzed in embryos (E), larval stages (L1-L4), young (YA) and gravid (GA) adults. Endogenous mature let-7 is present from L3 to GA (gradient shading). The black and light gray boxes represent stages with strong or weak GFP expression, respectively. Expression in the head and tail is seen in most neurons and muscle cells. Body wall muscle (BWM); ventral nerve cord (VNC); vulva includes precursor as well as mature cells; SEAM/ HYP, seam and hypodermal cells.