Fig. 7. The effect of promoter deletions on let-7 rescue activity.

(A) Constructs tested for let-7 rescue activity contained WT or the indicated deletions in the let-7 promoter sequences inserted into the mosSCI backbone to allow for single copy integration (ⁱ) into Chromosome II. (B) Rescue analysis was performed on integrated transgenic animals crossed to let-7(mn112) mutant animals. Viability was analyzed as the percent of animals alive at the early gravid adult stage. Shown is the average viability from three independent rescue experiments for each strain and n = the total number of animals analyzed.