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. Author manuscript; available in PMC: 2013 Jan 19.
Published in final edited form as: Biochemistry. 2012 Oct 19;51(43):8491–8501. doi: 10.1021/bi3010335

Table 2.

Presteady state parameters for correct incorporation of R and S CHF and CHCl diastereomers by R183A pol β. kpol and Kd values are reported as the mean ± standard error of three replicates.

Diastereomer kpol (s-1) Kd (μM)
(R)-CHF 0.27 ± 0.02 6.8 ± 1.0
(S)-CHF 0.29 ± 0.06 39 ± 8
(R)-CHCl 1.5 ± 0.1 110 ± 3
(S)-CHCl 0.88 ± 0.13 300 ± 96