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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Cancer Res. 2012 Dec 7;73(2):1000–1010. doi: 10.1158/0008-5472.CAN-12-3538

Fig. 5. DLX4 reduces levels of TOP2 poison-induced DSBs by stimulating Ku-dependent NHEJ activity.

Fig. 5

Cells of the Ku80-deficient cell line XR-V15B were transfected with Ku80 and/or DLX4. [A] Western blot of DLX4 and Ku80 levels in XR-V15B nuclear extracts. [B] Colony formation in end-joining assays using XR-V15B nuclear extracts. Shown are average results of 3 independent experiments. [C] DSBs were assayed in XR-V15B cells by neutral comet assays at 1 d after doxorubicin treatment (500 nM). Shown are mean tail moments of 3 independent assays where 50 randomly selected cells were scored in each assay.