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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Cancer Res. 2012 Nov 30;73(2):1011–1019. doi: 10.1158/0008-5472.CAN-12-3212

Figure 1. Apoptosis by extracellular Par-4 is regulated by NF-κB.

Figure 1

(A,B,C) IκB-SR up-regulates cell surface GRP78 and sensitizes cancer cells to apoptosis. Cells were transduced with IκB-SR or control GFP adenovirus; and then (B) unfixed cells were subjected to FACS analysis for cell surface GRP78, or (A, B) the cells were treated with (A) TRX-Par-4 or TRX (100 nM of each protein) or (B) GRP78 C-terminal agonistic antibody or PTEN control antibody (1 μg/ml) for 24 h. The cells were subjected to ICC for active caspase-3 and apoptotic cells were scored under a fluorescent microscope. Expression of IκB-SR was confirmed by Western blot analysis (A, inset).

(D) PS-1145 up-regulates cell surface GRP78 and sensitizes cells to apoptosis. Cells were co-treated with PS-1145 (10 μM) and TRX or TRX-Par-4 protein (100 nM), or GRP78 agonistic antibody or PTEN antibody (1 μg/ml) for 24 h, and subjected to FACS analysis for cell surface GRP78 (right panel) or to apoptotic assays (left panel).

Asterisk (*) indicates statistically significant (P < 0.001) difference by the Student t test, and (**) indicates that the effect is significantly (P < 0.001) higher based on two-way ANOVA with data normality and equality of variance assumptions.