(A) Egr-1 mRNA levels in scrambled siRNA transfected, Egr-1 and SREBP1c siRNA transfected cells exposed to zero (controls) or 50 mM EtOH for 24 hr in VL-17A cells. # vs †, P =0.0001; # vs ¶, P =0.0001. (B) SREBP1c mRNA levels in scrambled siRNA transfected, Egr-1and SREBP1c siRNA transfected cells exposed to zero (controls) or 50 mM EtOH for 24 hr in VL-17A cells. # vs †, P =0.002; # vs ¶, P =0.001. (C) Triglyceride levels in scrambled siRNA transfected, Egr-1and SREBP1c siRNA transfected cells exposed to zero (controls) or 50 mM EtOH for 24 hr in VL-17A cells. Data are mean values of 5 samples ± SEM. # vs †, P =0.00003. ****P < 0.0001, ***P < 0.001,**P < 0.01, *P < 0.05 (control vs ethanol).