Table 1.

Data collection and refinement statistics for BMP complexes of single mutants

	Q185A	R203A	T159V	T159A	T159S	R160A	Y206F	K82A
Data collection
Space group	P21	P21212	P21	P212121	P21	P21	P21	P21
No. of molecules	2	2	2	2	2	2	2	2
in asym. unit
Cell dimensions
a (Å)	59.80	80.00	59.78	52.61	58.67	59.80	59.62	59.78
b (Å)	63.86	64.00	64.12	74.04	73.87	64.14	63.41	64.01
c (Å)	61.47	73.30	61.89	117.50	59.52	61.78	61.04	61.63
β (°)	115.28		115.46		119.50	115.67	115.02	115.52
Resolution (Å)	1.37	1.50	1.40	1.60	1.3	1.4	1.42	1.49
No. of unique	87599	58327	79912	54292	104965	76701	76776	68389
reflections
Rmerge	0.065	0.082	0.091	0.076	0.038	0.083	0.073	0.043
Completeness (%)	99.7	95.6	95.8	88.5	96.8	91.5	98.1	99.7
Refinement
Resolution (Å)	25.0–1.37	25.0–1.3725.0	25.0–1.40	25.0–1.60	25.0–1.3	25.0–1.40	25.0–1.42	25–1.49
Rcryst	0.165	0.223	0.188	0.204	0.165	0.199	0.172	0.153
Rfree	0.178	0.247	0.206	0.231	0.176	0.222	0.193	0.174
No. atoms
Protein	3421	3412	3364	3322	3418	3374	3379	3416
Waters	454	144	396	170	410	410	347	444
Bound ligands	BMP SO4	BMP	BMP	BMP GOL	BMP GOL, POL	BMP	BMP GOL	BMP GOL
Ligand atoms	54	44	44	50	82	44	50	50
R.m.s. deviations
Bond lengths (Å)	0.006	0.005	0.006	0.007	0.006	0.006	0.006	0.006
Bond angles (°)	1.0	1.2	1.1	1.1	1.1	1.1	1.1	1.1
PDB entry	3V1P	3LI0	3P60	3P5Y	3P5Z	3P61	3RLV	3RLU