CEM cells were transduced with ZMIZ1-silencing shRNA. C-MYC expression was measured by Western blot (A) and by qPCR (B) relative to the level in non-transduced cells. (C) 8946 cells were transduced with the NGFR empty vector (open columns) or ZMIZ1 (black columns) and with the indicated NOTCH1 alleles. After 6 days of doxycycline treatment, the %NGFR+GFP+ cells was measured by flow cytometry and divided by the starting percentage prior to treatment to derive the fold increase in %NGFR+GFP+ cells. (D–H) Sorted 8946 cells were analyzed for c-Myc (D), Cad (E), Dtx1 (F), CD25 (G), and Hes1 (H) by qPCR. Expression is presented as a percentage of the value in ZMIZ1/L1601PΔP-transduced cells. (I–L) Sorted 8946 cells were treated with doxycycline. Open symbols represent 8946 cells co-transduced with the empty NGFR vector. Closed symbols represent 8946 cells co-transduced with ZMIZ1.