Figure 6. ZMIZ1 may regulate c-Myc through a novel MIZ-independent mechanism.

(A) Schematic diagram of the mutants of ZMIZ1 used in this experiment. (B) 8946 cells were transduced with the indicated ZMIZ1 constructs in the NGFR vector with the activated NOTCH1 allele L1601PΔP. After 6 days of doxycycline treatment, the absolute number of NGFR⁺GFP⁺ cells was counted and divided by the starting number to derive the absolute cell number fold increase. (C) Sorted NGFR⁺GFP⁺ 8946 cells were analyzed for c-Myc by qPCR relative to the NGFR vector. Control=8946 cells transduced with MigR1 and NGFR vectors. (D) Disordered profile plot of ZMIZ1 generated by DISOPRED2 (http://bioinf.cs.ucl.ac.uk/disopred/).