HOI-07 inhibits the anchorage-independent growth of a panel of NSCLC cell lines, including A549 cells (A), H1650 cells (B), and H520 cells (C). The asterisk indicates a significant (* p < 0.05, **p < 0.01) decrease in colony formation in cells treated with HOI-07 compared with the DMSO-treated group. D, HCT116 p53−/− cells are more sensitive to the effects of HOI-07 compared to wildtype HCT116 p53+/+ cells. The asterisk indicates a significant (* p < 0.05, **p < 0.01) decrease in anchorage dependent growth in HCT116 p53+/+ colon cancer cells compared to HCT p53−/− cells.