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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Cancer Res. 2012 Nov 1;73(2):716–724. doi: 10.1158/0008-5472.CAN-12-2784

Figure 3.

Figure 3

HOI-07 inhibits Aurora B kinase activity both in vitro and ex vivo. A, HOI-07 inhibits Aurora B in vitro kinase activity in a concentration-dependent manner. An inactive histone 3.3 protein was used as the substrate for an in vitro kinase assay with active Aurora B and 100 µM ATP. Proteins were resolved by SDS-PAGE and detected by Western blotting. B, HOI-07 has no effect on Aurora A in vitro kinase activity. C, HOI-07 inhibits phosphorylation of the Aurora B downstream target, histone H3 (Ser10) in human lung cancer cells in a time- (left) and dose-dependent (right) manner. A549 cells were treated with HOI-07 at the indicated concentration for 24 h or at 1 µM and harvested at the indicated time points. Cells were harvested and histone proteins were extracted and subjected to Western blot analysis.