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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Cancer Res. 2012 Nov 1;73(2):716–724. doi: 10.1158/0008-5472.CAN-12-2784

Figure 4.

Figure 4

HOI-07 induces polyploidy, apoptosis and G2/M phase cell cycle arrest in lung cancer cells. A, HOI-07 induces polyploidy in A549 cells. Cells were treated with HOI-07 (1 µM) for 48 h and then were evaluated by immunofluorescence assay. Scale bar indicates 20 µm (600X). B and C, HOI-07 induces G2/M arrest in lung cancer cells. Cell cycle analysis by flow cytometry of A549 cells (B) and H520 cells (C) treated with HOI-07. D and E, HOI-07 induces apoptosis in lung cancer cells. Flow cytometry analysis of apoptosis. A549 cells (D) and H520 cells (E) were incubated with the indicated concentration of HOI-07 for 72 h, then collected and apoptosis was detected by Annexin V and PI staining.