Representative photomicrographs of p-Akt (Ser473) ICC of hypothalamic sections at approximately bregma −4.16 (A–C) and −3.8 (D–F) from saline, leptin or insulin-treated rats that were fasted 24 hours and injected ICV with leptin (4 μg), insulin (10 mU) or saline and killed 45 min later. Scale bars, 100 μm. ARC, arcuate nucleus; PMv, ventral premammillary nucleus; VTM, ventral tuberomammillary nucleus; 3v, third ventricle.