Table 1.

Binding of rabbit GAPDH to several protoporphyrin IX analogs at pH 7.4 (Tris, 100 mM, 10% glycerol), room temperature.

	Wavelength maxima (nm)	Comments
Free porphyrin
Protoporphyrin IX (Porph IX)	347, 471, 539, 591, 642
Fe(III)-protoporph IX	352, 392, 497, 613	CT
Fe(III)-mesoporph IX	351, 389, 490, 604	CT
Fe(III)-deuteroporph IX	342, 386, 490, 602	CT
Fe(III)-2,4-diacetyldeuteroporph IX	342, 403, 426 (s), 625	CT
Co(III)-protoporph IX	351, 417, 531, 565	LS
Zn(II)-protoporph IX	347 (s), 393, 550, 589	LS
GAPDH-porphyrin complex
Protoporph IX	347, 471, 539, 591, 642	No binding
Fe(III)-protoporph IX	366, 412, 536	LS, red-shift
Fe(III)-mesoporph IX	353, 403, 533	LS, red-shift
Fe(III)-deuteroporph IX	344, 404, 531	LS, red-shift
Fe(III)-2,4-diacetyldeuteroporph IX	347, 404, 428, 443 (s)	LS, partial binding, red-shift
Co(III)-protoporph IX	352, 426, 536, 568	LS, red-shift
Zn(II)-protoporph IX	343 (s), 393, 420, 549, 588	LS, partial binding, red-shift