Table 2.
Kinetic parameters of heme binding proteins with various functions.
| Protein | kon (M−1s−1) | koff (s−1) | Kd (koff/kon) (M) |
Kd (direct) (M) |
Function | Reference |
|---|---|---|---|---|---|---|
| Wild type rGAPDH | 1.78 × 104 | 3.3 × 10−4 7.0 × 10−3 |
19 × 10−9 39 × 10−8 |
24 × 10−9 | Unknown | This work |
| Nuclear hormone receptor, Rev-erb β (oxidized) | ND | ND | ND | 1.17 × 10−7 | Gene repression; circadian cycle, inflammation | (14) |
| Nuclear hormone receptor, Rev-erb β (reduced) | ND | ND | ND | 23 × 10−9 | Gene repression; circadian cycle, inflammation | (14) |
| Sperm whale Mb | 7.6 × 107 | 8.4 × 10−7 | 1.1 ×10−14 | ND | O2 transport | (56) |
| HRI (heme regulated inhibitor), eIF2α | 1.1 × 107 | 1.5 × 10−3 | 1.4 × 10−10 | ND | Regulation of protein synthesis by iron | (60) |
| BSA | 5.0 × 107 | 1.1 × 10−2 | 2.2 × 10−10 | ND | Fatty acid transport; heme metabolism | (56) |
| Apo-DHR51-LBD (D. melanogaster, homologous of human nuclear receptor Reverb α and β | ND | ND | ND | 4.3 × 10−7 | Nuclear receptor | (81) |
| CcmE | ND | ND | ND | 2 × 10−7 (high affinity) 10 × 10−6 (low affinity) |
Heme chaperone; cytochrome c biogenesis in Gram-negative bacteria | (82) |
| HasAp | 1.6 × 107 | ND | 35 ×10−6 | ND | Heme acquisition from Hb in P. aeruginosa | (34) |
| HtsA | 8 × 107 | ND | 8 ×10−6 | ND | Heme acquisition by S. pyogenes | (83) |
| Shp | 1.6 × 106 | ND | 22 × 10−6 | ND | Cell surface hemin transfer protein in S. pyogenes | (84) |
| HemQ | ND | ND | ND | 30–40 | Heme synthesis by M. tuberculosis | (71) |
| HTP (PhuT) | ND | 3.8 × 10−4 | ND | 0.0012 | Heme acquisition by P. aeruginosa | (21) |
| PhuS | 1.8 × 105 | 3.6 × 10−2 | 0.2 ×10−6 | ND | Heme acquisition by P. aeruginosa | (57) |
| pa-HO | 1.1 × 105 | 6.6 × 10−2 | 0.6 × 10−6 | ND | Heme degradation by P. aeruginosa | (57) |
| Rv0203 | 1.3 × 108 | 8.2 ×10−2 | 6.2 × 10−10 | ND | Heme binding protein M.tuberculosis | (85) |