Skip to main content
. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Cancer Res. 2012 Nov 7;73(2):695–705. doi: 10.1158/0008-5472.CAN-12-2522

Figure 1. Myc induces PTEN expression and inhibition of the PI3K/Akt pathway resulting in increased active Ezh2.

Figure 1

(A) Immunoblot of PTEN protein levels in rat fibroblasts with or without Myc and in IMECs with or without exogenous Myc.

(B) RT-PCR of PTEN RNA levels in rat fibroblasts with or without Myc and in IMECs with or without exogenous Myc. * denotes p-value < 0.05, # denotes p-value < 0.01

(C) Immunoblot analysis of the indicated proteins in myc−/− fibroblasts and IMECs expressing empty vector or exogenous Myc. Experiments were performed three or more times and representative blots are shown.

(D) siRNA-mediated depletion of PTEN leads to enhanced pEzh2 and pAKT in IMECs.

(E) IMECs as in (C) were treated with LY294002 for 2 hours or left untreated. Cells were harvested and lysates were immunoblotted with the indicated antibodies. Inhibition of the PI3K/Akt axis suppresses pAkt (P-S473) and pEzh2 (P-S21 Ezh2).