Fig. 2.

BCR-ABL expression enhances inflammatory mediator gene expression. (A) Bright-field (BF) and fluorescent (GFP) microscopy showing cord blood-derived colonies transduced with lentiviral vector backbone control (pCDH) or lentivirus expressing BCR-ABL p210 and GFP. (B) Nanoproteomics analysis of phosphorylated (p)-Crkl levels in BCR-ABL–transduced CD34⁺ cells from cord blood (n = 3). P < 0.05 by Student t test. (C) CD34⁺ cord blood cells (n = 3) were transduced with lenti-BCR-ABL or vector control and processed for RNA-seq analysis. Forty-five genes were differentially expressed in BCR-ABL–expressing cells and vector controls (P < 0.05 by DESeq).