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. 2012 Dec 27;110(3):948–953. doi: 10.1073/pnas.1208286110

Fig. 2.

Fig. 2.

L2 region strongly influences [2Fe-2S] cluster transfer function of mitoNEET and cluster stability. (A) Transfer of the [2Fe-2S] cluster from mitoNEET and L2 mutants to apo-ferredoxin. Cluster transfer experiments were performed aerobically at 37 °C at pH 8.0 using 100 μM mitoNEET or L2 mutants and 100 μM apo-ferredoxin in 50 mM Bis-Tris and 100 mM NaCl. (B) Stability of mitoNEET’s [2Fe-2S] cluster compared with L2 mutants. Cluster stability measurements were performed aerobically at 37 °C. Loss of the [2Fe-2S] cluster was measured over time as a decrease in absorbance at 458 nm. Studies were performed using 40 μM mitoNEET or L2 mutants in 100 mM Bis-Tris and 100 mM NaCl, pH 7.0. WT is shown in black, and controls are shown in gray.