Selective depletion of IM in DT-treated CD11b-DTR BM-chimeric mice. (A) Spleen cells from CD11b-DTR BM-chimeric mice, with or without injection of DT 24 h earlier, were stained with PE–anti-Ly6C plus Alexa Fluor 700–anti-CD11b or PE–anti-Ly6G plus Alexa Fluor 700–anti-CD11b. Numbers represent percentages of total spleen cell population (average of three mice per group). (B) Spleen cells obtained as in (A) were stained with various combinations of mAbs, and cell types were identified as indicated in text. Numbers represent percentages of total spleen cell population (average of three mice per group). (C) Peritoneal cells were stained with various combinations of mAbs, and B cell subsets were identified as indicated. Numbers represent percentages of total peritoneal cell population (average of three mice/group). One of two representative experiments. *p ≤ 0.05. cDC, Conventional DC; FOB, follicular B; pDC, plasmacytoid DC.